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. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: Methods Mol Biol. 2019;1982:191–229. doi: 10.1007/978-1-4939-9424-3_12

Fig. 4.

Fig. 4

Example of a RT-qPCR standard curve used for the determination of primer set efficiency. Ten tenfold serial dilutions were made from pcDNA3.1-NOX4 variant 1 at a starting concentration of 1 μg/μL. Primers (200 nM) for NOX4 (E4F and E5R) from Table 2 were used. The Cq values were plotted vs. log (dilution-fold), and the efficiency of the primer set was determined from the slope