Table 10.
DUOX antibodies
| DUOX1, DUOX2, DUOXA1, DUOXA2 source/catalog # | Host and clonality | Immunogen (aa) | Is the target detected in WB (other applications)? | Species detected (human, mouse, rat) | Reference |
|---|---|---|---|---|---|
| DUOX1 and 2 Dr. U. Knaus |
Rabbit Polyclonal |
DUOX1 775–1026 |
Yesa (Flow, IHC) |
H, M | [27] |
| DUOX2 specific Dr. U. Knaus |
Rabbit Polyclonal |
634–648 | Yes (Flow, IHC) |
H | [28] |
| DUOXA1 specific Dr. U. Knaus |
Rabbit Polyclonal |
280–299 | Yes | H | [28] |
| DUOX1 and 2 Dr. X. De Deken |
Rabbit Polyclonal I2 |
DUOX1 618–1004 |
Yesa | H, M | [29] |
| DUOX1 and 2 Dr. C. Dupuy |
Rabbit Polyclonal |
DUOX2 410–423 |
Yesa | H, M | [30] |
| DUOX1 specific Dr. C. Dupuy |
Rabbit Polyclonal |
DUOX1 988–1011 |
Yes | H, M | [31] |
| DUOX1 and 2 Dr. C. Dupuy |
Mouse Monoclonal Clone |
DUOX2 21–589 |
Yesa | H | [32] |
| DUOX1 and 2 Dr. J. Doroshow |
Mouse Monoclonal Clone S-40 |
DUOX2 131–540 |
Yesa | H, M | [33] |
| DUOX1 Bioss bs-11431R |
Rabbit Polyclonal |
Unspecified | Nob | H, M, R | |
| DUOX2 Santa Cruz (Y-15) sc-49939 |
Goat Polyclonal |
Unspecified | Yesa | H, M, R | |
| DUOX2 Santa Cruz (E-8) sc-398681 |
Mouse Monoclonal |
DUOX2 626–680 |
Not testedc | H, M, R | |
| DUOX1 Sigma-Aldrich HPA023544 |
Polyclonal | 910–1011 | Yesa (IHC) |
H | |
| DUOXA1 specific Sigma-Aldrich HPA041578 |
Polyclonal | 270–326 | Yesd (IHC) |
H |
Evaluation of DUOX1, DUOX2, DUOXA1, and DUOXA2 antibodies
a
Cross-reactivity between DUOX1 and DUOX2. Dr. Dupuy’s monoclonal antibody has ~tenfold preference for DUOX2, and in porcine tissues this antibody does not cross-react with Duox1 (see reference [32])
b
Detects a non-specific band at a similar molecular weight as DUOX in non-transfected cells
c
Recommended by Santa Cruz as a replacement for Santa Cruz (Y-15) sc-49939. See website for images
d
Detects additional non-specific bands