Table 11.
Antibodies for subunits (p47phox, p67phox) and for p22phox
| Subunits and p22phox source/catalog # | Host and Clonality | Immunogen (aa) | Is the target detected in WB (other applications)? | Species detected (human, mouse, rat) | Reference |
|---|---|---|---|---|---|
| p47phox Dr. M. Quinn Millipore 07–001 |
Rabbit Polyclonal |
Full-length human p47phox | Yesa, b (IHC, IP) |
H, M, R | [34] |
| p47phox Dr. M. Quinn |
Mouse Monoclonal Clone 43.12 |
Full-length human p47phox | Yes (others not tested) | H, M, R | Unpublished |
| p67phox Dr. M. Quinn Millipore 07–002 |
Rabbit Polyclonal |
Full-length human p67phox | Yesa, b (IHC, IP) |
H, M, R | [34] |
| p67phox Dr. M. Quinn |
Mouse Monoclonal Clone 81.1 |
Full-length human p67phox | Yes (IHC) | H, M, R | Unpublished |
| p47phox Santa Cruz sc-17844 | Mouse Monoclonal Clone A-7 |
196–390 | Yesa (others not tested) | H, M, R. | |
| p67phox Santa Cruz sc-374510 | Mouse Monoclonal Clone D-6 |
Full-length p67phox | Yesa (others not tested) | H, M, R | |
| p40phox Dr. M. Quinn |
Mouse Monoclonal Clone 1.9 |
Full-length human p40phox | Yes (IHC) | H | |
| p22phox Dr. A. Jesaitis Dr. M. Quinn Santa Cruz sc-130550 |
Mouse Monoclonal Clone 44.1 |
29–33 and 182–188 |
Yesa, b (IP) |
H, M, R | [14] |
Many academic laboratories have published antibodies generated for these subunits. We have used antibodies generated in the laboratory of Dr. Mark Quinn
Also listed are commercial antibodies that worked in our conditions for immunoblots
a
Tested with human neutrophil cytosolic fractions
b
Tested in HEK 293 cells overexpressing cytosolic components