Table 5.

NOX1 and NOXA1 antibodies

NOX1 source/catalog #	Host and clonality	Immunogen (aa)	Is the target detected in WB (other applications)?	Species detected (human, mouse, rat)	Reference
Dr. J. Doroshow	Mouse Monoclonal Clone 22	224–564	Yesa (IF, IHC)	H	[11, 12]
Dr. C. Yabe-Nishimura	Rabbit Polyclonal	139–152 (mouse)	Yesb (IF, IHC)	M	[7]
Dr. C. Yabe-Nishimura	Rat Monoclonal Clone 31C1	139–152 (mouse)	Yesb (IF, IHC)	M	Unpublished
Dr. J. David Lambeth	Mouse Monoclonal Clone E39	3rd extracellular loop	Yesc (IF, IHC)	H	[13]
OriGene Technologies #TA349080	Rabbit Polyclonal	Proprietary	Nod	H
Novus #NBP1–31546	Rabbit Polyclonal	Proprietary	Nod	H, M, R
NOXA1 subunit Santa Cruz sc-398873	Mouse Monoclonal Clone H-6	1–200	Yese	H, M, R

Evaluation of academic and commercial NOX1 antibodies

^aSpecificity in cell lines is shown in Ref. [11]; specific in CHO cells overexpressing NOX1 (Dr. V. Jaquet, pers. communication)

^bAuthors stated that the polyclonal antibody detected Nox1 in mouse colon but not in mouse small intestine or lung. Their mAb detects Nox1 in mouse colon, small intestine, and lung (personal communication Dr. M. Matsumoto) (see Fig. 8)

^cThere was a limited quantity of this antibody available for WB verification (see Fig. 5d). This antibody was made by diaDexus (out of business). The specific epitope was not disclosed, only the location (3rd extracellular loop)

^dUnable to differentiate between NOX1 stably overexpressed in HEK 293 cells and empty vector-transfected controls

^eProduct datasheet indicates that NOXA1 was detected in HEK 293T cells overexpressing NOXA1, but not in vector control