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. 2021 Jan 19;2(3):100164. doi: 10.1016/j.xplc.2021.100164

Figure 1.

Figure 1

Fine mapping of Xa7.

(A) Physical map of Xa7 locus and associated markers on chromosome 6. The individual molecular markers are indicated as the genomic coordinates (in kilobases and in green) on chromosome 6 of the Nipponbare reference genome.

(B) Genomic regions delimited by two markers (M5 and M5-48k) in Nipponbare and IRBB7. Annotated genes in Nipponbare are shown with red arrows, and those in IRBB7 are shown in blue. Red bars linking Nipponbare and IRBB7 indicate syntenic regions. Two Cas9 guide RNAs for the deletion of a 53-kb region within the Xa7 candidate gene in IRBB7 are denoted by red arrows.

(C) Schematic graph of Xa7. The gene structure is depicted as EBE for AvrXa7, 5′ UTR for the 5′ untranslated region, and 3′ UTR for the 3′ untranslated region. CDS, coding sequence. (D) XA7 amino acids. Single letters of amino acids are used. The transmembrane sequences are underlined.