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. 2021 Jan 19;2(3):100164. doi: 10.1016/j.xplc.2021.100164

Figure 3.

Figure 3

Disease phenotypes of Xa7 mutant lines.

(A) Genotypes of three CRISPR lines with deletions and insertions at two guide RNA target sites. Guide RNA target sequences are shaded, and the adjacent Cas9 PAM sequences are underlined. Start codons are in red and bold, and stop codons are in red. Inserted nucleotides are in bold lowercase letters, and dashed lines indicate deleted nucleotides.

(B) Genotypes of three CRISPR lines with Xa7 knocked out. Predicted amino acids encoded by different alleles are shown, with WT sequences in bold and new amino acids due to frameshift mutations underlined.

(C) The isogenic line NB7 and three CRISPR lines containing Xa7 knockout mutations were inoculated with PXO86. Lesion lengths were measured as for Figure 2. Treatments with the same lowercase letter are not significantly different at p < 0.05.