RT mixture
| Reagent | Volume (μL) |
|---|---|
| 5× Maxima RT buffer | 50 |
| RNase-free water | 32.8 |
| RNase Inhibitor (Enzymatics) | 1.6 |
| Superase•In RNase Inhibitor (Ambion) | 3.2 |
| dNTPs (10 mM stock) | 12.5 |
| Maxima H Minus Reverse Transcriptase | 25 |
| PBS-RI | 100 |
| Total | 225.1 |
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