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. 2021 Jan 9;2(3):100143. doi: 10.1016/j.xplc.2021.100143

Figure 2.

Figure 2

CRISPR-Cas9-mediated knockout of Xa7.

(A) The CRISPR-Cas9 editing sites of targets 1 and 2 in Xa7. The blue box represents the exon of Xa7. The sgRNA sequences of targets 1 and 2 are shown in green, and the protospacer adjacent motif (PAM) is shown in red.

(B and C) Sequences of targets 1 (B) and 2 (C) in the CRISPR-Cas9 transgenic lines of rice variety Zhen-hui 084 (ZH084). Wild type refers to the sequence of ZH084. ko-1 to ko-4 refer to the different types of knockout mutants. The sgRNA sequence regions are shown in green, and mutations are shown in red. Numbers in red are the base-mutation changes in the single chromosome, and base deletions and insertions are indicated with minus and plus, respectively.

(D) Leaves of the ko1 to ko4 mutants of Xa7 and the wild-type control ZH084 inoculated with Xoo strain PXO86. Photographs were taken 2 weeks after inoculation. Bar, 2 cm.