Figure 3.

Nodulation phenotypes of sunn-6 and nlp1-1 grafted plants.

(A–C) The grafted plants were grown in nitrogen-starved substrate and irrigated with 10 mM KCl or KNO₃. Total nodules and large nodules were counted at 3 wpi. Nodule phenotypes (A), total nodule number (B), and number of big nodules (≥1 mm) (C) in grafting combinations of sunn-6 and nlp1-1.

(D) Acetylene reduction assay (nM min⁻¹ g nodule⁻¹) of nodules from different grafting combinations of sunn-6 and nlp1-1 grown in nitrogen-free substrate and irrigated with 10 mM KCl or KNO₃ (n = 3). Scale bar in (A), 1 mm; error bars show ±SEM. Data points from one representative experiment of two independent experiments are plotted as dots. Asterisks indicate significant differences (two-tailed t-test, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; ns, not significant).