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. 2021 May;191(5):885–901. doi: 10.1016/j.ajpath.2021.02.008

Supplemental Figure S1.

Supplemental Figure S1

AAV8-TBG-Cre–driven hepatocyte-specific knockdown of β-catenin and γ-catenin [double knockout (DKO)] causes liver injury. A: Schematic showing delivery of AAV8-TBG-Cre or AAV8-TBG-GFP to β-catenin; γ-catenin double-floxed mice to generate β-catenin; γ-catenin DKO or control (CON), respectively. B: Serum analysis from control (AAV8-GFP) and AAV8 Cre β-catenin; γ-catenin mice indicates persistent increases in direct and total bilirubin and alkaline phosphatase, starting from day 12 after injection. C: Immunohistochemistry for β-catenin, depicting its intact expression in both hepatocytes and cholangiocytes in CON livers, whereas in DKO livers, hepatocytes are completely devoid of β-catenin staining, and cholangiocytes are β-catenin positive. D: Scatterplot of the RNA-sequencing data analysis between DKO and CON livers harvested from β-catenin; γ-catenin double-floxed mice 12 days after AAV8-TBG-Cre or AAV8-TBG-GFP injection. Scale bars: 5 μm (C, left column); 10 μm (C, right column).