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. 2020 Oct 31;599(1):143–155. doi: 10.1113/JP279810

Figure 1. Validation of mechanically‐regulated miRs in cartilage explants using qPCR.

Figure 1

qPCR validation of mechanically‐regulated miRs, identified by NGS, in cartilage explants subjected to loads of 2.5 or 7 MPa (1 Hz, 15 min) and analysed 24 h post‐cessation of load for (A) miR‐21‐5p, (B) miR‐27a‐5p, (C) miR‐221, (D) miR‐222, (E) miR‐451, (F) miR‐483 and (G) miR‐453; unloaded explants served as controls. miR levels were normalized to the geometric mean of two reference genes (SDHA, YWHAZ) and further normalized relative to the unloaded control cDNAs. Data are presented as box plots depicting the mean ± 95% CI (n = 6 explants) and are representative of three independent experiments. Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test.