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. 2020 Oct 31;599(1):143–155. doi: 10.1113/JP279810

Figure 3. Validation of TIMP3 and CPEB3, putative target genes of miR‐221, miR‐222 or miR‐21, using TaqMan qPCR.

Figure 3

Primary bovine chondrocytes were treated with either 50 nm miR mimic, 50 nm inhibitor or negative control siRNAs for each respective miR for 48 h, prior to analysis of the effect of overexpression and knockdown of miR‐221 on TIMP3 transcription (A and B), miR‐222 on TIMP3 transcription (C and D), and miR‐21 on TIMP3 (E and F) and CPEB3 (G and H) transcript levels after normalization to the geometric mean of the reference genes HPRT and YWHAZ and further normalization to respective negative control siRNAs. Data are presented as the mean ± 95% CI (n = 3 wells) and are representative of three independent experiments. Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test.