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. 2020 Oct 31;599(1):143–155. doi: 10.1113/JP279810

Table 1.

Sequences of primers used to clone the 3′‐UTR of mRNAs containing the predicted binding site of target miRs

Target 5′‐ to 3′ Oligo sequence Annealing temperature (°C)
TIMP3 UTR F 5′‐GCTCGCTAGCCTCGACTGAGCTTCCCTTGGACACT‐3′ R 5′‐CGACTCTAGACTCGAGCTAAAGGGAAAGGCGGAT‐3′ 60
CPEB3 UTR F 5′‐GCTCGCTAGCCTCGAAAGGAGGGAAAAGAGAGGGC‐3′ R 5′‐CGACTCTAGACTCGAAACAGAGCACCGCAAAGTAC‐3′ 60