Table 2.

Mean fold‐change and statistical significance of mechanically‐regulated miRs in articular chondrocytes subjected to loads of 2.5 or 7 MPa (1 Hz, 15 min), to represent a physiological or non‐physiological load respectively, and analysed 2, 6 and 24 h post‐cessation of load (unloaded explants served as controls)

	UL vs. 2.5MPa		UL vs. 7MPa		2.5 vs. 7MPa
	FC	Padj	FC	Padj	FC	Padj
Analysed 2 h post‐cessation of load
miR‐27a‐5p	4.420	2.79 × 10–22	7.092	3.08 × 10–40
miR‐2898	0.588	0.008	0.408	3.16 × 10–10
miR‐2478			0.566	0.001
miR‐98			1.698	0.001
miR‐23b‐3p			1.539	0.004
miR‐1260b			0.647	0.025
miR‐23a			1.464	0.034
miR‐148b			1.644	0.039
Analysed 6 h post‐cessation of load
miR‐486	0.521	0.002
miR‐677			2.149	5.50 × 10–5	1.933	0.001
miR‐222			1.568	0.008
miR‐2889					2.393	1.54 × 10–6
miR‐1249					0.562	0.013
Analysed 24 h post‐cessation of load
miR‐222	1.814	0.003	7.409	8.81 × 10–51	4.085	4.00 × 10–25
miR‐27a‐5p			3.019	1.14 × 10–16	2.027	5.84 × 10–7
miR‐221			3.393	1.63 × 10–13	2.593	3.58 × 10–8
miR‐543			2.619	1.30 × 10–9	1.725	0.005
miR‐21‐5p			2.267	6.67 × 10–6	1.719	0.013
miR‐495			1.775	1.45 × 10–4
miR‐451			0.481	0.002
miR‐425‐5p			0.626	0.010	0.672	0.037
miR‐20a			1.603	0.012
miR‐7			1.699	0.017
miR‐760‐3p			1.715	0.017
miR‐2318			1.8518	0.022
miR‐2344			1.829	0.030
miR‐431			1.817	0.030
miR‐155			1.472	0.042
miR‐100			1.429	0.048
miR‐483					0.599	0.025

Data are representative of three independent experiments (n = 6 explants per individual experiment).