Table 2.
Reagents used for OMIP‐069
| Specificity | Fluorochrome | Clone | Purpose |
|---|---|---|---|
| Viability | Live Dead UV Blue | — | Viability |
| CD45 | PerCP | HI30 | Leukocytes |
| CD3 | BV510 | SK7 | Pan T cell, NKT‐Like cells |
| CD4 | cFluor YG584 | SK3 | CD4 T and NKT‐Like cells |
| CD8 | BUV805 | SK1 | CD8 T, NK, and NKT‐Like cells |
| CD25 | PE‐Alexa Fluor700 | CD25‐3G10 | Regulatory T cells |
| TCRγδ | PerCP‐eFluor 710 | B1.1 | Pan γδ T cell |
| CD14 | Spark Blue 550 | 63D3 | Monocyte differentiation |
| CD16 | BUV496 | 3G8 | Monocyte, NK cell, and dendritic cell differentiation |
| CD11c | eFluor 450 | 3.9 | Dendritic Cell differentiation |
| CD19 | Spark NIR 685 | HIB19 | B cells |
| CD20 | Pacific Orange | HI47 | B cells |
| CD24 | PE‐Alexa Fluor 610 | SN3 | B cell differentiation |
| CD39 | BUV661 | TU66 | B cell, Tregs, and monocyte differentiation |
| IgD | BV480 | IA6‐2 | B cell differentiation |
| IgG | BV605 | G18‐145 | B cell differentiation |
| IgM | BV570 | MHM‐88 | B cell differentiation |
| CD141 | BB515 | 1A4 | Dendritic cell differentiation |
| CD1c | Alexa Fluor 647 | L161 | Dendritic cells, NKT‐Like cells |
| CD123 | Super Bright 436 | 6H6 | Plasmacytoid dendritic cells |
| CD2 | PerCP‐Cy5.5 | TS1/8 | NK cell differentiation |
| CD56 | BUV737 | NCAM16.2 | Pan NK cell, γδ T cell activation |
| CCR7 | BV421 | G043H7 | T cell differentiation |
| CD27 | APC‐H7 | M‐T271 | T and B cell differentiation |
| CD28 | BV650 | CD28.2 | T cell and NK cell differentiation |
| CD45RA | BUV395 | 5H9 | T cell and dendritic cell differentiation |
| CD95 | PE‐Cy5 | DX2 | T cell and B cell differentiation |
| CD127 | APC‐R700 | HIL‐7R‐M21 | Cytokine receptor; T cell differentiation |
| CD337 | PE‐Dazzle594 | P30‐15 | NK cell differentiation |
| CCR6 | BV711 | G034E3 | Chemokine receptor; T cell and B cell differentiation |
| CCR5 | BUV563 | 2D7/CCR5 | Chemokine receptor; Monocyte, dendritic cell, T cell, and B cell differentiation |
| CXCR5 | BV750 | RF8B2 | Chemokine receptor; T cell differentiation |
| CXCR3 | PE‐Cy7 | G025H7 | Chemokine receptor; Dendritic cell, T cell, and B cell differentiation |
| HLA‐DR | PE‐Fire810 | L243 | T cell and monocyte activation, NK cell lineage discrimination, dendritic cell lineage marker |
| CD38 | APC‐Fire810 | HIT2 | Monocyte, dendritic cell, T cell, and B cell activation/differentiation |
| CD57 | FITC | HNK‐1 | NK and CD8+ T cell immune senescence |
| PD‐1 | BV785 | EH12.2H7 | T cell inhibitory receptor |
| CD159a | APC | REA110 | NK, NKT‐Like, and γδ T cell activation/differentiation |
| CD159c | PE | REA205 | NK cell differentiation |
| CD314 | BUV615 | 1D11 | NK cell differentiation |