Fig. 2.
Effect of inducing A. baumannii CptA and CptB on the growth of E. coli. (A) Growth curves of E. coli Bl21(DE3) cells harboring the dual promoter plasmid pBAD18-cptA-cptB-PT7, induced by either 0.2% L-arabinose (CptA inducer) or IPTG 1 mM (CptB inducer) vs. uninduced cells, those carrying pBAD18 ori (control vector), and the untransformed wild-type bacterial cells. Samples were drawn every hour for 10 h. (B) Bar chart representing the viable counts of E. coli Bl21(DE3) cells carrying pBAD18-cptA-cptB-PT7 upon induction with 0.2% L-arabinose or IPTG 1 mM, compared to uninduced cells, those with vector alone (pBAD18 ori), and untransformed wild-type cells. Viable counts samples were collected at different time points, serially diluted and plated on either LBA or LBA with apramycin. The viable counts were calculated as CFU/ml and Log10 data plotted against time. The data represent the means of three independent experiments. The error bars represent the standard error of the means. Asterisks (*) indicate statistically significant differences (at p < 0.05), in comparison to uninduced cells as determined by ANOVA followed by post hoc t-test.