Table 1.
List of plasmids used in this study.
| Plasmids | Characterization | Source/ Reference |
|---|---|---|
| pET28b (+) | Expression vector with IPTG-inducible promoter, T7 (PT7) and kanamycin resistant gene, kanR | Department of Microbiology plasmid collection/ Novagen, USA |
| pBAD18 ori | Expression vector with apramycin resistant gene, ApmR. Modified form of pBAD18 plasmid, with 1337 bp PCR product containing the replication origin of cryptic plasmid pWH1277 from Acinetobacter lwoffii DSM30013 | Dr. Stephen Lory, Harvard Medical School |
| pBAD42 | Expression vector with arabinose-inducible promoter, araBAD (PBAD) and spectinomycin/streptomycin resistant gene, spR/smR | [41] |
| pET28b-cptB-cptA | Derivative of pET28b containing cptA-cptB where the cptB antitoxin gene is under the control of the PT7 promoter; kanR | This study |
| pBAD18-cptA-cptB-PT7 | Derivative of pBAD18 containing cptA-cptB-PT7 promoter and lacI gene where the cptA toxin gene become under the control of the arabinose pBAD promoter; apmR | This study |
| pBAD42-cptBA | Derivative of pBAD42 containing cptBA under the control of the arabinose pBAD promoter, smR | This study |