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. 2021 May 18;12:2931. doi: 10.1038/s41467-021-23212-x

Fig. 6. Ex vivo IAV- and IBV-specific CD8+ T-cells detected in HLA-A24+ PBMCs and tissues.

Fig. 6

a Tetramer-positive cells of three different specificities (A/PB1498–505, B/NP165–173, B/NA32–40) were enriched in human blood and tissues. Representative FACS plots of enriched fractions are shown for healthy donors, acute influenza-infected patients, and tissues from deceased organ donors. b Tetramer+ precursor frequencies in the blood of healthy LIFT and non-LIFT, and influenza-infected donors where acute and convalescent time-points are shown together (IBV-inf). Samples from donor IBV2-inf were collected at acute square and convalescent timepoint triangle. c Activation status of epitope-specific CD8+ T-cells in healthy, acute and convalescent donors. Statistical significance was calculated using a two-tailed Mann–Whitney test. d T-cell differentiation phenotype of epitope-specific cells in healthy LIFT and non-LIFT donors, and influenza-infected patients. e Tetramer+ precursor frequencies of epitope-specific cells in human tissues. f CD103 and CD69 expression on epitope-specific cells in the lung compared to secondary lymphoid organs (SLO; CD8+ T cells 2 spleens, 1 lymph node, 3 tonsils; A/PB1498-specific cells 2 spleens, 2 tonsils; B/NP165-specific cells 2 spleens, 1 lymph node, 2 tonsils). b, e Lines represent the median. c, d, f Bars represent mean + SD.