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. 2021 May 19;12:292. doi: 10.1186/s13287-021-02348-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Characterization of the HHEX KO pig embryos compared to WT. a Phenotype of the HHEX KO E18 pigs. b, c IHC images of E18 HHEX KO pig embryos with Hoechst (blue), anti-AFP/HHEX (red), and anti-FOXA2/cTnT (green) antibodies. White insets in b correspond to numbered enlarged images in c. Bars: all are 100 μm, except for the bar in a, which is 500 μm. d–h Gene expression analyses (RNA-seq, d–g; qRT-PC, h) in the HHEX KO E18 pigs vs WT (N = 3 each group). d Principal component analysis. e Heatmap showing all the significantly up- and downregulated genes. f Heatmap showing liver-related genes whose expressions are significantly up- or downregulated. g Volcano plot showing significantly up- and downregulated genes (both in blue) and unaffected genes (orange), highlighting some liver-related genes. h Relative expression of transcripts. **p < 0.01; **p < 0.001