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. 2021 May 14;14:1781–1794. doi: 10.2147/IDR.S304864

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© 2021 Wang et al.

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Effects of complex on osteogenic marker collagen I and osteocalcin of four groups of cells were analyzed by Real-time PCR and Western blot in vitro.

Notes: (A) The expressions of osteogenic marker genes collagen I and osteocalcin were conducted by Real-time PCR. (B) The protein expressions of collagen I and osteocalcin in BMSCs were detected by Western blot. (C) The relative intensity of each band was quantitated using ImageJ software. GAPDH was employed as a loading control. Above results were obtained from three independent experiments. The data were presented as mean ± SD. **vs the BMSCs group, **P<0.01; ^{^^}vs the IBMSCs group, ^{^^}P<0.01; ^♯♯vs complex group, ^♯♯P<0.01.

Abbreviations: BMSCs, bone marrow mesenchymal stem cells; IBMSCs, induced bone marrow mesenchymal stem cells; RPSMs, rifapentine polylactic acid sustained-release microspheres; HA/β-TCP, hydroxyapatite/β- tricalcium phosphate.