Skip to main content
. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: Cell Rep. 2021 Apr 6;35(1):108964. doi: 10.1016/j.celrep.2021.108964

Figure 7. Arid1b is indispensable for OD differentiation but is not involved in Hh signaling regulation.

Figure 7.

(A and B) Immunofluorescence of Arid1b of first mandibular molars of control and Gli1-CreER;Arid1afl/fl mice at PN7.5.

(C) Western blot of Arid1b in the apical region of the first mandibular molars from control and Gli1-CreER;Arid1afl/fl mice at PN7.5.

(D) UCSC Genome Browser screenshot of Arid1b at the Gli1 locus and ChIP assay with Arid1b antibody (or IgG), followed by qPCR. n = 3, *p < 0.05.

(E) Western blot of Gli1 and Ccnd1 in DPCs with control siRNA or four different Arid1b siRNA sets.

(F) Western blot of Gli1 and Ccnd1 in DPCs with control siRNA, Arid1a siRNA, or both Arid1a and Arid1b siRNA.

(G) Western blot of Dspp and Dmp1 in DPCs with control siRNA or four different Arid1b siRNA after odontogenic induction.

(H) Alizarin red S staining of DPCs with control siRNA or four different Arid1b siRNA after odontogenic induction. Schematic indicates induction protocol. Scale bars: 100 μm.