KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-ARID1A | Abcam | Cat# ab182561 |
| Rabbit anti-Ki67 | Abcam | Cat# ab15580, RRID:AB_443209 |
| Chicken anti-beta Galactosidase | Abcam | Cat# ab9361, RRID:AB_307210 |
| Rabbit anti-K14 | Abcam | Cat# ab181595, RRID:AB_2811031 |
| Rabbit anti-phospho-Histone H3 (Ser10) | Millipore | Cat# 06-570, RRID:AB_310177 |
| Rabbit anti-Cyclin D1 | Abcam | Cat# ab16663, RRID:AB_443423 |
| Rabbit polyclonal anti-Periostin | Abcam | Cat# ab14041, RRID:AB_2299859 |
| Rabbit anti-Arid1b | Abcam | Cat# ab244351 |
| Mouse monoclonal anti-ARID1A | Santa Cruz | Cat# sc-32761, RRID:AB_673396 |
| Mouse monoclonal anti-ZAC1(Plagl1) | Santa Cruz | Cat# sc-166944, RRID:AB_10613622 |
| Rabbit anti-Gli1 | Novus | Cat# NBP1-78259, RRID:AB_11030198 |
| Mouse monoclonal anti-DSPP | Santa Cruz | Cat# sc-73632, RRID:AB_2230660 |
| Sheep anti-DMP-1 | R&D | Cat# AF4386, RRID:AB_2091367 |
| Mouse monoclonal anti-cyclin D1 | Santa Cruz | sc-8396, RRID:AB_627344 |
| Mouse monoclonal anti-beta Actin | Abcam | Cat# ab20272, RRID:AB_445482 |
| Rabbit anti-ARID1A/BAF250A (D2A8U) | Cell Signaling Technology | Cat# 12354, RRID:AB_2637010 |
| Rabbit anti-ARID1B/BAF250B (E9J4T) | Cell Signaling Technology | Cat# 92964, RRID:AB_2800195 |
| Normal Rabbit IgG | Cell Signaling Technology | Cat# 2729, RRID:AB_1031062 |
| Mouse IgG HRP-conjugated antibody | R&D | Cat# HAF007, RRID:AB_357234 |
| Rabbit IgG HRP-conjugated antibody | R&D | Cat# HAF008, RRID:AB_357235 |
| Sheep IgG HRP-conjugated antibody | R&D | Cat# HAF016, RRID:AB_562591 |
| Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11008, RRID:AB_143165 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A-21207, RRID:AB_141637 |
| Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11039, RRID:AB_142924 |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat# T5648-5G |
| Rodent Diet (2018, 625 Dox, R) | ENVIGO | Cat# TD.08541 |
| Fetal Bovine Serum | Thermo Fisher Scientific | Cat# 12662029 |
| α-MEM | Thermo Fisher Scientific | Cat# 12571071 |
| Collagenase, Type 1 | Worthington | Cat# LS004194 |
| RIPA Buffer (10X) | Cell Signaling Technology | Cat# 9806s |
| Protease inhibitor | ThermoFisher Scientific | Cat# A32959 |
| DAPI Solution (1 mg/mL) | Thermo Fisher Scientific | Cat# 62248 |
| Alizarin Red S | ACROS Organics | Cat# 400480250 |
| Critical commercial assays | ||
| Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection, Alexa Fluor 488 dye | Thermo Fisher Scientific | Cat# C10617 |
| Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye | Thermo Fisher Scientific | Cat# C10637 |
| RNeasy Micro Kit | QIAGEN | Cat# 74004 |
| RNAscope® Multiplex Fluorescent v2 | ACD | Cat# 323100 |
| RNAscope 2.5 HD Reagent Kit-RED assay | ACD | Cat# 322350 |
| Probe- Mm- Gli1 | ACD | Cat# 311001 |
| Probe- Mm- Gli1-C2 | ACD | Cat# 311001-C2 |
| Probe- Mm- Ptch1 | ACD | Cat# 402811 |
| Probe- Mm- Plagl1 | ACD | Cat# 462941 |
| Probe- Mm- Dspp | ACD | Cat# 448301 |
| Probe- Mm- Axin2 | ACD | Cat# 400331 |
| Probe- Mm- Lef1 | ACD | Cat# 441861 |
| iScript cDNA Synthesis Kit | Bio-Rad | Cat# 1708891 |
| SsoAdvanced Universal SYBR® Green Supermix | Bio-Rad | Cat# 1725270 |
| SimpleChIP® Plus Enzymatic Chromatin IP Kit | Cell Signaling Technology | Cat# 9005 |
| Deposited data | ||
| Bulk RNA-seq data | This paper | GEO: GSE166360 |
| ATAC-seq data | This paper | GEO: GSE166360 |
| Arid1b ChIP-Seq data | This paper | GEO: GSE166360 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | Jackson Laboratory | JAX:000664, RRID:IMSR_JAX:000664 |
| Mouse: Arid1afl/fl:STOCK Arid1atm1, 1Zhwa | Jackson Laboratory | JAX:027717, RRID:IMSR_JAX:027717 |
| Mouse: Gli1-CreER: STOCK Gli1tm3(cre/ERT2)Alj | Jackson Laboratory | JAX:007913, RRID:IMSR_JAX:007913 |
| Mouse: Gli1-LacZ:STOCK Gli1tm2Alj/J | Jackson Laboratory | JAX:008211, RRID:IMSR_JAX:008211 |
| Mouse: tdT:ROSA26loxp-STOP-loxp-tdTomato:B6;129S6-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J | Jackson Laboratory | JAX:007905, RRID:IMSR_JAX:007905 |
| Mouse: K14-rtTA:B6;SJL-Tg(KRT14-rtTA) 208Jek | Jackson Laboratory | JAX:007678, RRID:IMSR_JAX:007678 |
| Mouse: tetO-cre: B6.Cg-Tg(tetO-cre)1Jaw/J | Jackson Laboratory | JAX:006234, RRID:IMSR_JAX:006234 |
| Mouse: Dmp1-Cre: B6N.FVB-Tg (Dmp1-cre)1Jqfe/Bwd | Jackson Laboratory | JAX:023047, RRID:IMSR_JAX:023047 |
| Mouse: SmoM2fl/fl | (Jeong et al., 2004) | N/A |
| Oligonucleotides | ||
| Primer sequences | See Table S2 | N/A |
| Software and algorithms | ||
| ImageJ | NIH | RRID:SCR_003070 |
| GraphPad Prism | GraphPad Software | RRID:SCR_002798 |