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. 2021 May 5;12:643275. doi: 10.3389/fmicb.2021.643275

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Copyright © 2021 Pietschmann, Voepel, Voß, Rasche, Schubert, Kleines, Krause, Shaw, Spiegel and Schroeper.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Magnetic immunodetection-based SARS-CoV-2-specific antibody titer determination. Four previously tested positive and three negatively tested patient sera were analyzed in serial dilution starting from 20-fold up to 2560-fold dilution in phosphate-buffered saline (PBS). Samples were applied onto ready-to-use immunofiltration columns (RTU-IFCs) followed by application of biotinylated anti-human IgG-specific secondary antibody. Finally, magnetic particles functionalized with streptavidin were applied, and measuring signal was determined using portable frequency mixing magnetic detection (FMMD) readout device. Each data point represents n = 1. Dashed line represents the threshold.