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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Acta Biomater. 2020 Nov 6;119:184–196. doi: 10.1016/j.actbio.2020.11.007

Figure 2. Marker expression of XF-hiPSC-ECs.

Figure 2.

(A) Immunostaining of EC markers (CD31, VECAD, eNOS and vWF) and pluripotency marker OCT4 in XF-hiPSC-ECs, XG-hiPSC-ECs, XF-HUVECs, XG-HUVECs, and undifferentiated hiPSCs under xenogeneic-free conditions. DNA (nuclear) was counterstained by DAPI. Scale bar: 200 μm. (B) Percentage of cells (XF-hiPSC-ECs, XG-hiPSC-ECs, XF-HUVECs, XG-HUVECs, and xenogeneic-free hiPSCs) positive for EC markers (CD31, VECAD, eNOS and vWF) and pluripotency marker OCT4 from immunostaining (One-way ANOVA with Tukey’s multiple comparisons test; Mean values and S.E.M indicated by error bars are shown; n=3; ****: p<0.00 01; N.S: not significant).