Table 1.

The primer-probe sets used in this study.

Genotype	Primer-probe set	Name	Sequence	Amplicon size
c.851_854	1	1F	5′-GCCAAACTGAAGGCTATACTG-3′	164 bp
		1R	5′-CTCTTCCAGAGGAGCAAT-3′
		1P	5′-FAM-TTTGTTTTTCCCCTACAGACGTATGACCTTAGCA-BHQ1-3′
c.1638_1660dup	2	2F	5′-GACTGAGATGGTGTTGTGT-3′	124 bp
		2R	5′-ACTCCGCTGTAAGTGGTT-3′
		2P	5′-CY5-ACGAGATTACTGGTGGCTGCCCGGGGAGATTACAG-BHQ2-3′
IVS6+5G>A	3	3F	5′-CGACTTCCGAGACATCAT-3′	278 bp
		3R	5′-ATTCCGTATTACCCAGACAA-3′
		3P	5′-CY5-AGTAGCTGTAAGTTGTAAC-BHQ2-3′
IVS16ins3kb	4	4F	5′-ATACTGCGTGAAGAAGGAC-3′	291 bp (Wild) 447 bp (Mutation)
		4R1	5′-CTACGACAACAGAGCATTAG-3′
		4R2	5′-CCCTCACTGCTGATTCTTAGATAG-3′
		4P1	5′-ROX-CAGATTTAGCATGATACTTACACTCCTC-BHQ2-3′
		4P2	5′-ROX-TTCTTTATATTTGATAGACTGC-BHQ2-3′