FIGURE 3.
The effect of cell washing and cell recovery on CFT. 1 × 106 viable MSCs were isolated for inclusion in each respective group. Recovered cells were cultured for 24 hours and dissociated from the culture plate immediately prior to use. A, Cells collected immediately after thaw and cells collected after recovery were subjected to staining and flow cytometry. Each curve represents the outcome of three pooled samples. B, Direct injection groups were either thawed directly into plasma, washed with saline, or recovered with 24 hours of culture at 37°C. Microreactor groups were seeded with MSCs and either immediately used or allowed to attach for 2 hours at 37°C followed by a 24‐hour hold at room temperature prior to perfusion. Values for CFT were determined and resulting values were graphed and analyzed with an unpaired Student's t test. N = 3 runs per group. ****P < .0001. Error bars represent ± SD