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. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: ACS Biomater Sci Eng. 2020 Feb 11;6(3):1690–1703. doi: 10.1021/acsbiomaterials.9b01837

Figure 4. Proteomics and ECM- effect on ACTA2 and Col1A1 expression.

Figure 4.

Discovery proteomics for VFLP-ECM and UBM-ECM. A) Venn diagram grouping and contrasting the total number of proteins detected and identified for each biomaterial. B) Volcano plot representing the significant ECM related proteins (gene name nomenclature) after Perseus analysis. Proteins displayed were detected in at least two independent replicates in either VFLP or UBM ECM. C) Heat map for the entire set of ECM related proteins detected in either scaffold. The (+) or (−) symbol next to the gene name indicates if the protein was or was not significant in the relative quantification between VFLP and UBM ECM. D) Volcano plot showing the proteins related to the TGF-β1 pathway. E) Fold Change of ACTA2 and Col1A1 in reference to TGF-β1 untreated. Error bars represent SEM. UBM-ECMh condition treated and untreated had a p-value <0.05 for ACTA2 analyzed by t-test paired with at least n=5.