A.) ECM scaffolds were enzymatically digested and self-assembled into ECM hydrogels; human dermal fibroblasts (HDFn) were seeded on top and cytocompatibility determined. B.) dsDNA quantification of HDFn cultured on Col control, mH-ECMh-O, sH-ECMh, and tissue culture plastic (TCP). * = P≤0.05. Bar graphs represent mean ± SEM. C.) LIVE/DEAD imaging of HDFn cells cultured on TCP, Col control, mH-ECMh-O, and sH-ECMh. Calcein-AM staining (Green) shows live cells while Ethidium Homodimer-1 staining (Red) shows dead cells (Scale bar = 330μm).