Table 1.

Steps in the 3-hour automated decellularization of the porcine heart including reagent and exposure time.

#	Reagent	Time (minutes)
1	DI Water	5
2	2X DPBS	5
3	0.02% Trypsin	30
4	DI Water	5
5	2X DPBS	5
6	3% Tween-20	30
7	DI Water	5
8	2X DPBS	5
9	4% Sodium Deoxycholate	30
10	DI Water	5
11	2X DPBS	5
12	0.1% Peracetic Acid	30
13	1X DPBS	15
14	1X DPBS	15