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Steps in the 2.5-hours automated decellularization of porcine VFLP including reagent and exposure time.
| # | Reagent | Time (minutes) |
|---|---|---|
| 1 | DI Water | 5 |
| 2 | 2X DPBS | 5 |
| 3 | 4% Sodium Deoxycholate | 30 |
| 4 | DI Water | 5 |
| 5 | 2X DPBS | 5 |
| 6 | DNAse (273Kunitz/mL) | 30 |
| 7 | DI Water | 5 |
| 8 | 2X DPBS | 5 |
| 9 | 0.1% Peracetic Acid | 30 |
| 10 | 1X DPBS | 15 |
| 11 | 1X DPBS | 15 |
