Figure 2.

Hyperoside application causes increased fruit size and seed set. A, Surface views and longitudinal sections of representative okra fruits from the control, buffer, and hyperoside treatment groups at 16 DAA. Bar=1 cm. B, Characteristics of fruit from the three treatment groups: average weight per fruit, fruit length, fruit width, seed set, number of seeds per fruit, weight per thousand seeds, and seed size. Error bars show the standard error of the mean (sem; n = three biological replicates). *P < 0.05 (Student’s t test). C, The contents of total protein, total fat, total polysaccharide, and total flavonoids in seeds DAA16 for the control, buffer, and hyperoside treatment groups. Different letters (a and b) indicate significant differences (P<0.05) between genotypes using Duncan’s multiple range test after analysis of variance (ANOVA). D, HPLC profiles of fruit from the three treatment groups. 1–6 indicate the reference standards: 1. Rutin, 2. Isoquercetin, 3. Hyperoside, 4. Myricetin, 5. Quercetin-3-O-glucoside, 6. Quercetin. E, The content of six flavonoids in fruit from the three treatment groups, analyzed with standards. Error bars show the standard error of the mean (sem; n = three biological replicates). *P < 0.05 (Student’s t test). FW, fresh weight