Figure 2.

Characterization of C-terminal GFP fusions of ascorbate biosynthesis enzymes. A, The ascorbate complementation assay rescues growth arrest from gldh and lgaldh mutants but not gme lethality. Insets show homozygous mutants with arrested growth in media lacking ascorbate, but not in media supplemented with ascorbate. HZ, heterozygous. B, Complementation of ascorbate concentration by expression of GFP fusion proteins in ascorbate deficient Arabidopsis mutants. Data displayed correspond to mean±SD from three independent samples composed of three 6-week-old fully expanded rosettes grown in a short-day regime at 150-µmol photons m⁻² s⁻¹, one and a half hours after lights turned on. Different letters denote statistically significant differences for ascorbate concentration (one-way ANOVA, α = 0.05, post-hoc Tukey test). C, Immunoblot (α-GFP) of complemented lines shows that a small amount of GGP compared with other components restores ascorbate content. Red arrowhead indicates GGP-GFP. CBB, Coomassie Brilliant Blue.