Figure 5.

Subcellular localization of ascorbate biosynthesis enzymes fused to GFP at the C-terminus and expressed under the control of their native promoters in Arabidopsis. A, The expression of GFP-tagged enzymes is ubiquitous in 4-d-old seedlings and their subcellular localization is compatible with the cytosol and nucleus except for GME, which only locates in cytosol. B, GFP signal of a functional GGP-GFP (line 13) protein was not detectable. GFP was visualized by laser scanning confocal microscopy. Scale bar = 30 µm.