Skip to main content
. 2021 Jan 25;185(4):1574–1594. doi: 10.1093/plphys/kiab010

Figure 7.

Figure 7

The enzymes of the Smirnoff-Wheeler pathway elute together in high-molecular weight fractions of N. benthamiana protein extract. A, All the proteins transiently expressed were separated in 1 mL fractions and mainly accumulated between 393 and 48 kDa. B, Longer exposure of the blot presented in (A) shows that all the proteins eluted in high-molecular weight fractions. C, Fractions collected every 0.5 mL more accurately determined that the accumulation of proteins mainly occurs between 81 and 138 kDa but a longer exposure (D) enabled the detection of proteins in higher-molecular weight fractions than that. Proteins from N. benthamiana leaves were extracted as described in “Co-IP assay” section and separated as described in “Gel filtration chromatography” section. T=Total protein extract; MW=molecular weight. Calculated MWs were estimated using Dextran blue (2,000 kDa), Alcohol dehydrogenase (150 kDa), Albumin (66 kDa), and Carbonic Anhydrase (29 kDa); GLDH* points to a degradation product of GLDH (see Supplemental Figure S9).