Skip to main content
. 2020 Dec 7;185(2):405–423. doi: 10.1093/plphys/kiaa051

Figure 9.

Figure 9

RALF peptide treatment terminates root hair growth in the FER(WT)-EYFP expressing line. (A) Root hair tip-growth in stably transformed lines expressing the FER(WT)-EYFP fluorescent fusion protein under permissive (20°C) temperature by time-lapse microscopy. Bright field images of growing root hair cells (n = 3) were collected using a Nikon Eclipse E600 wide-field microscope with a ×20 Plan Apo (0.45 NA) lens with 5 s intervals. The dashed line represents when media containing RALF1 (1 µM) was perfused into the growth chamber. Representative images are presented at 200 s intervals. Scale bar: 50 µm. (B) Quantification of root hair growth. Root hair elongation was measured every 50 s using the measurement function in ImageJ. Error bars represent sd.