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. 2020 Nov 28;185(1):210–227. doi: 10.1093/plphys/kiaa009

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© The Author(s) 2020. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Spectroscopic analysis of thylakoids isolated from Columbia-0 (Col-0) and carotenoid-deficient Arabidopsis (A. thaliana) plants grown in LL and adapted to EL conditions. A, B, CD spectra normalized to the maximum of the Chl Q_y absorption band. C, D, Low temperature (77 K) chlorophyll fluorescence emission spectra normalized to equal area under the curve. E, F, Chlorophyll fluorescence emission difference spectra mutant-minus-Col-0 in LL conditions (E) and EL–LL for all genotypes (F). G, H, Laurdan fluorescence emission spectra normalized to the maximal value. I, Laurdan GP values for LL and EL samples. All presented spectra are representative of three independent experiments; pairs of results marked with asterisk differ significantly at p = 0.05 (one-way ANOVA with post hoc Tukey test; n ≥ 4).