Figure 4.

Kinetics of photoprotective gene expression under different light conditions. RNA was extracted from wild-type cell samples collected after 0, 15, 30, 60, 120, and 240 min of irradiation with low-level UV-supplemented fluorescent light at 10 μmol photons/m²/s (Supplemental Figure S3), blue (470-nm) LED light, or red (660-nm) LED light at 110 μmol photons/m²/s, as in Figure 3. Expression levels of the photoprotection-related PSBS1, LHCSR1, and LHCSR3.2 genes were analyzed using RT-qPCR. The color bars represent expression levels of each gene in UV light- (purple bar), blue light- (blue bar), and red light- (red bar) treated cells. The CBLP housekeeping gene was used as a control. Data are mean ± se, n = 3 biological replicates. Statistical significance was analyzed with multiple t tests using Benjamini, Krieger and Yekutieli’s two-stage false discovery rate method procedure, with Q = 1%; *** denotes P < 0.001; ** denotes P < 0.01; *denotes P < 0.05; n.s. = not significant.