Figure 5.

Photoprotective gene expression in the uvr8 mutant under light of different qualities. RNA and protein were extracted from samples of control (CS; LHCSR1–Luc717) and uvr8-mutant strains illuminated for 1 h with low-level UV-supplemented fluorescent light at 10 μmol photons/m²/s (Supplemental Figure S3), blue (470 nm) LED light, or red (660 nm) LED light at 110 μmol photons/m²/s, as indicated, and compared with control samples maintained under LL (∼30 μmol photons/m²/s). A, Expression levels of the PSBS1, LHCSR1, and LHCSR3.2 genes related to photoprotection were analyzed using semi-quantitative RT-PCR. The CBLP gene was used as a loading control. B, LHCSR1 and LHCSR3 protein levels were detected using an antibody against LHCSRs (recognizing both LHCSR1 and LHCSR3). LHCSR3-P represents LHCSR3-phosphorylated. The ATPB protein was used as a loading control. Representative gels and immunoblots from one of three replicated experiments are shown, each performed using different biological samples.