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. 2021 Jan 13;185(4):1894–1902. doi: 10.1093/plphys/kiab004

Figure 6.

Figure 6

Photoprotective response of UV-treated cells. Cells of control (LHCSR1–Luc717), uvr8-mutant, and complemented (uvr8/UVR8–FLAG) strains were treated with low-level UV-supplemented fluorescent light (10 μmol photons/m2/s) for 1 h to induce photoprotective mechanisms and compared with control samples maintained under LL (∼30 μmol photons/m2/s). A, UVR8–Venus–FLAG and UVR8 proteins were detected using antibodies against FLAG and CrUVR8, respectively. LHCSR1 and LHCSR3 protein levels were detected using an antibody against LHCSRs (recognizing both LHCSR1 and LHCSR3). The ATPB protein was used as a loading control. B, NPQ was recorded using a moni-PAM system (Walz). A light-response curve was generated from measurements at 0, 45, 90, 190, 420, 820, and 1,500 μmol photons/m2/s. Glycolaldehyde at 10 mM final concentration was added 3 min before the measurements to interrupt the Calvin–Benson–Bassam cycle. Data are mean ± se, n = 3 biological replicates.