Figure 2.

DRP1A contributes positively to plant immunity against Pto DC3000 bacteria. A and B, Leaves of 5-week-old Col-0, drp1a^salk, drp1a^rsw9, and drp2b-2 plants were syringe-infiltrated with (A) virulent Pto DC3000 (OD₆₀₀ = 0.0005); or (B) hypovirulent Pto DC3000 hrcC^- (OD₆₀₀ = 0.02). Bacterial growth was assessed by serial dilution plating as cfu at 0 and 3 d post-infection (dpi). For 3 dpi, n = 8 different plants/genotype, and for 0 dpi, n = 4 different plants/genotype; with each n consisting of three leaf discs taken from three different leaves/plant for each genotype and time-point. Values are means ± se with different letters indicating statistically significant differences and with same letters indicating no statistically significant differences based on ordinary one-way ANOVA (P < 0.0001). All experiments were performed at least three times with similar results using biologically distinct samples for each biological replicate. dpi, days post-infection; OD, optical density. Col-0, WT (white bar); 2b-2, drp2b-2 (gray bar); 1a^rsw9, drp1a^rsw9 (black bar); 1a^salk, drp1a⁰⁶⁹⁰⁷⁷ (gray bar with black stripes).