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. 2021 Jan 5;185(4):1411–1428. doi: 10.1093/plphys/kiaa048

Figure 1.

Figure 1

ShHTL7 has moderate affinity for SL but high affinity for AtMAX2. (A) Dissociation constants (Kd) for 5DS binding to each ShHTL protein determined by ITC. Data are means sd (n = 3). (B) In vitro pull-down assays of the interactions between each ShHTL protein and AtMAX2 in the absence or presence of 1 μM 5DS or rac-GR24. The GST-AtMAX2 bait protein was visualized by staining the PVDF membrane with Memstain to show equal loading. The ShHTL proteins with His6-Flag tag were detected with anti-Flag antibody.