Figure 3.

shSGD does not deglycosylate strictosidine. A, SDS-PAGE analysis of the purified SGD and shSGD. B, Time-course analysis of a representative strictosidine deglycosylation conducted by incubating 50 µM of strictosidine with SGD or shSGD. C, MIAs formed through deglycosylation of strictosidine by SGD. 1, epi-cathenamine; 2, dehydrogeissoschizine; 3, cathenamine. D, The aglycone crosslinking product resulting from SGD activity. E, Coomassie blue staining and F, zymogram of SGD and shSGD activities. Non-heat-treated recombinant proteins were separated on a semi-native PAGE before incubation in a MUGlc containing solution and UV-visualization.