Figure 5.

In vitro licheninase activity test of maize MLGH1 paralogs. A, Table showing the activity of MLGH1, MLGH1^E262K mutant version (mlgh1-1), and five selected maize paralogs. b-1,3-1,4: licheninase activity using MLG as substrate. b-1,3: 1,3-b-glucanase activity using laminarin as substrate. b-1,4: 1,4-b-glucanase activity using cellooligosaccharides as substrate. Data shown in nKat/mg. n.d. = no activity was detected. B, Detail of the maximum-likelihood phylogenetic tree of MLGH1 and its closest paralogs in maize. See Supplemental Figure S7 for the complete phylogenetic tree.