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. 2020 Dec 4;185(2):533–546. doi: 10.1093/plphys/kiaa046

Figure 2.

Figure 2

ABA-stimulated phosphorylation of Ser1029 detected by anti-phosphopeptide antibody. (A) Proteins from protonemata of transgenic P. patens AR7 lines expressing ARK-GFP (ARK) and ARK-GFP with S532F, S1029A, and S1030A mutations with or without treatment with 10-µM ABA for 15 min were subjected to immunoblot analysis using an anti-phospho-Ser1029 (P-S1029) antibody and an anti-GFP antibody. Since levels of ARK-GFP accumulation vary among these lines, the amount of proteins loaded per lane was adjusted using the anti-GFP antibody. Note that ARKS1030A-GFP tends to give enhanced signals for an unknown reason. (B) Detection of native ARK in WT P. patens. Protonemata were treated with 10-µM ABA for the indicated time periods, and extracted proteins were reacted with the anti-P-S1029 antibody and an antibody that recognizes the C-terminal 15-amino-acid peptide of ARK (anti-ARKc).