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. 2020 Dec 4;185(2):533–546. doi: 10.1093/plphys/kiaa046

Figure 5.

Figure 5

ABA response of the ppabi1 line of P. patens. (A) Growth responses to ABA in protonemata of WT and ppabi1. The protonemata were grown on medium with or without 10-µM ABA for 1 week. (B) Effect of ABA treatment on Ser-1029 phosphorylation in WT and ppabi1. Protonemata were treated with 10-µM ABA for different times and used for immunoblot analysis with anti-ARKc and anti-phospho-Ser1029 (P-S1029) antibodies. Coomassie Brilliant Blue staining of the large subunit of ribulose bisphosphate carboxylase (rbcL) and SnRK2 activity analyzed by an in-gel kinase are also shown.