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. 2020 Dec 3;185(2):457–468. doi: 10.1093/plphys/kiaa036

Figure 4.

Figure 4

Expression and protein accumulation pattern of OsVST1 are consistent with mutant RSA phenotype of E104-SR. (A) GUS staining of root systems of ProVST1:GUS transgenic seedlings at 2, 4, 6, 8, and 10 ds after germination. (B)–(F) GUS staining of 5-d-old ProVST1:GUS transgenic seedlings. Stem base (B), root tip (C), primary root with lateral roots (D), cross section of the primary root maturation zone (E), and root tip costained with safranin (F). (G)–(I) Colocalization of GFP-VST1 protein with the plasma membrane marker FM4-64 in primary root cells. GFP-VST1 (G), FM4-64 (H), and merged image (I). Bars, 3 cm in (A); 1 mm in (B)–(D); 100 μm in (E); 200 μm in (F); and 20 μm in (G)–(I). Inset image in (F) is enlarged 300x.