Figure 1.

3-D reconstructions of S. hermonthica and P. japonicum haustoria. A, Representative image of S. hermonthica 2 weeks after infection of a rice root. A magnified image of a terminal haustorium is shown in the right panel. B, Representative image of a P. japonicum root 9 d after infection of an Arabidopsis root. Right panel shows the magnified image of a lateral haustorium. Scale bars: left panels in A and B = 1 mm; right panels in A and B = 500 µm. C, Workflow of 3-D reconstruction. 1. Tissue preparation, sectioning, staining, photographing, and aligning image position. 2. Manual painting of each cell type in every fifth image. 3. Extraction of cell outline in all sections. 4. Segmentation of each cell in all sections. 5. Automated color coding of the unpainted images, based on manually painted images using automated interpolation. 6. Reconstruction of 3-D haustorial structure.