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. 2020 Nov 23;185(1):228–239. doi: 10.1093/plphys/kiaa019

Figure 2.

Figure 2

Transcriptional regulation of the daytime peaking GA biosynthetic genes, KS and GA20ox1. (A) The role of the circadian clock in mediating rhythmic expression. Light gray boxes represent subjective night. Sampling started the second day after beginning continuous light conditions. (B) Effect of a 3-h early start of day. Dark and light gray represent night and the subjective night, respectively. DCMU and mock solution (100 µM) was applied at zeitgeber time 12. Ctrl: 12L/12D, ed: early start of day. (C) The effect of reduced light availability or DCMU (NL + dcmu) on KS and GA20ox1 transcript abundance. DCMU was applied at the start of the preceding night. All data are from ∼10 leaf stage soil grown Col-0 rosettes. Means ± SE are shown, n = 4. Asterisks (planned comparisons) and letters (Tukey honestly significant difference (HSD)) represent statistically significant difference at specific time points, P < 0.05).