Increase of circadian period due to Mg deficiency is light dependent. (A) Fresh weight (FW) biomass (mean ± SEM, n = 3–4 [1 = 4 pooled seedlings]) of seedlings entrained to SD 8-/16-h light/dark cycles and LD 16-/8-h light/dark cycles. pCCA1:LUC average normalized luminescence traces of seedlings entrained for eight days to (B) SD, (C) LD on Mg-sufficient (200 µM) and deficient (5 µM) medium before released into LL, (D) RAE of SD and LD (mean ± SEM, n = 12–48). Circadian period of SD and LD and respective RAE changes significantly between 200-µM and 5-µM Mg (P < 0.001). (E) pCCA1:LUC average normalized luminescence traces of seedlings entrained for 8 d to a 12-/12-h light/dark cycles on 200-µM and 5-µM Mg before released into DD, (F) respective RAE. Significance between Mg concentrations was verified by two-sample Student’s t test at 95% CI (***P < 0.001).