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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Curr Opin Endocr Metab Res. 2021 Mar 20;18:118–127. doi: 10.1016/j.coemr.2021.03.005

Table 3.

Recent Transcriptomic and Proteomic Studies providing insight into Primordial Follicle Formation

Transcriptomics Proteomics
Method:
RNA sequencing on mouse ovaries		 Method:
LCMS/MS, GO and KEGG pathway analysis on mouse ovaries
Results:

  • Figla deficiency alters expression of genes involved in meiosis and oogenesis.

  • Figla deficiency disrupts oocyte specific expression of transcriptional regulators.

  • Lhx8 deficiency disrupts oogenesis-associated genes.

  • Figla, Lhx8, and Sohlh1 nulls contain significant overlap in down regulated genes in the mouse ovary.

 Results:

  • ZP2, ZP3, PAD16, FIGLA and OOEP were upregulated during primordial follicle activation.

  • Energy metabolism may be related to primordial follicle formation and activation.

  • Protein degradation is inhibited and protein synthesis is increased during primordial follicle formation and activation.
Reference: [43] Reference: [52]
Method:
SC-RNA sequencing on mouse pre-granulosa cells.		 Method:
2D-DIGE and MALDI-TOF MS on pig ovaries
Results:

  • Bipotential derived pregranulosa cells (BPG) were located in the medullar region and are thought to assist in rapid direct follicle development.

  • BPG cells were shown to express more androgen degrading enzymes

  • Follicles in the cortex contain epithelial derived pregranulosa (EPG) cell populations differentiate around 14.5 dpc.

 Results:

  • Members of the CREC family as well as RNC1 and RCN3 regulate primordial follicle formation

  • Actin and HNRNPK are upregulated during follicle formation

  • HSPA2 and gelsolin are upregulated during follicle activation.
Reference: [51] Reference: [53]